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A simple colorimetric method for viable bacteria detection based on cell counting Kit-8
In this study, Cell Counting Kit-8 (CCK-8) was introduced to detect the concentration of live bacteria for the first time depending on the redox reaction between CCK-8 solution and dehydrogenase. CCK-8 solution can be reduced to form water soluble orange-yellow formazan by the dehydrogenase present in bacterial cells, and the concentration of live bacteria is …
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Ceruloplasmin Deficiency Impaired Brain Iron Metabolism and Behavior in Mice
Iron accumulation is an important cause of various brain diseases. As a ferroxidase, ceruloplasmin (Cp) plays a key role in iron homeostasis and its abnormal activity leads to iron accumulation. However, the detailed biological function of Cp in brain iron homeostasis needs to be investigated. In this study, Cp knockout mice were prepared and the …
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Photoproduction of catalase-insensitive peroxides on the donor side of manganese-depleted photosystem II: evidence with a specific fluorescent probe.
The photoproduction of organic peroxides (ROOH) in photosystem II (PSII) membranes was studied using the fluorescent probe Spy-HP. Two types of peroxide, highly lipophilic ones and relatively hydrophilic ones, were distinguished by the rate of reaction with Spy-HP; the former oxidized Spy-HP to the higher fluorescent form Spy-HPOx within 5 min, while the latter did …
A simple colorimetric method for viable bacteria detection based on cell counting Kit-8
In this study, Cell Counting Kit-8 (CCK-8) was introduced to detect the concentration of live bacteria for the first time depending on the redox reaction between CCK-8 solution and dehydrogenase. CCK-8 solution can be reduced to form water soluble orange-yellow formazan by the dehydrogenase present in bacterial cells, and the concentration of live bacteria is …
A simple colorimetric method for viable bacteria detection based on cell counting Kit-8 Read More »
Efficient immobilization of catalase on mesoporous MIL-101 (Cr) and its catalytic activity assay
Enzyme immobilization using metal-organic frameworks (MOFs) as carriers has aroused significant interest owing to the unique pore structure and versatile surface functional groups of MOFs. Catalase (CAT) is an important industrial enzyme that is widely used in the catalytic decomposition of hydrogen peroxide in the fields of food and biological products. In this study, mesoporous …
Synergetic integration of catalase and Fe 3 O 4 magnetic nanoparticles with metal organic framework for colorimetric detection of phenol
Toxic phenol pollutants pose a great threat to the environment, it is urgent to develop an efficient and recyclable method to monitor phenol. Herein, we reported the synthesis of [email protected] ([email protected]) through a novel amino-acid-boosted one-pot embedding strategy that synergically integrated catalase and magnetic Fe3O4 nanoparticles with ZIF-8. As expected, CAT-Fe3[email protected] exhibited enhanced catalytic activity compared …
3D origami paper-based ratiometric fluorescent microfluidic device for visual point-of-care detection of alkaline phosphatase and butyrylcholinesterase
On-site multiplex enzyme detection is crucial for diagnosis, therapeutics and prognostic. To date, it is still a daunting challenge to develop portable, low-cost, and efficient multi-enzyme detection methods. Herein, a novel sample-in-result-out platform integrating ratiometric fluorescent assays with 3D origami microfluidic paper-based device (μPAD) was developed for simultaneous visual point-of-care testing (POCT) of alkaline phosphatase …
Pandora box of BCA assay. Investigation of the accuracy and linearity of the microplate bicinchoninic protein assay: Analytical challenges and method modifications to minimize systematic errors
Bicinchoninic colorimetric assay is very widely used for total protein quantitative analysis. We report that bicinchoninic (BCA) total protein assay linearity range and the assay sensitivity are counterbalancing factors. BCA assay true linear range may be considerably narrower than the manufacturer’s advertised 20-2000 μg/ml and therefore the choice of the assay calibration range should not …
Pandora box of BCA assay. Investigation of the accuracy and linearity of the microplate bicinchoninic protein assay: Analytical challenges and method modifications to minimize systematic errors
Bicinchoninic colorimetric assay is very widely used for total protein quantitative analysis. We report that bicinchoninic (BCA) total protein assay linearity range and the assay sensitivity are counterbalancing factors. BCA assay true linear range may be considerably narrower than the manufacturer’s advertised 20-2000 μg/ml and therefore the choice of the assay calibration range should not …