National surveillance for non-polio enteroviruses in Canada: Why is it important?

A simple colorimetric method for viable bacteria detection based on cell counting Kit-8

In this study, Cell Counting Kit-8 (CCK-8) was introduced to detect the concentration of live bacteria for the first time depending on the redox reaction between CCK-8 solution and dehydrogenase. CCK-8 solution can be reduced to form water soluble orange-yellow formazan by the dehydrogenase present in bacterial cells, and the concentration of live bacteria is …

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Photoproduction of catalase-insensitive peroxides on the donor side of manganese-depleted photosystem II: evidence with a specific fluorescent probe.

The photoproduction of organic peroxides (ROOH) in photosystem II (PSII) membranes was studied using the fluorescent probe Spy-HP. Two types of peroxide, highly lipophilic ones and relatively hydrophilic ones, were distinguished by the rate of reaction with Spy-HP; the former oxidized Spy-HP to the higher fluorescent form Spy-HPOx within 5 min, while the latter did …

Photoproduction of catalase-insensitive peroxides on the donor side of manganese-depleted photosystem II: evidence with a specific fluorescent probe. Read More »

A simple colorimetric method for viable bacteria detection based on cell counting Kit-8

In this study, Cell Counting Kit-8 (CCK-8) was introduced to detect the concentration of live bacteria for the first time depending on the redox reaction between CCK-8 solution and dehydrogenase. CCK-8 solution can be reduced to form water soluble orange-yellow formazan by the dehydrogenase present in bacterial cells, and the concentration of live bacteria is …

A simple colorimetric method for viable bacteria detection based on cell counting Kit-8 Read More »

Synergetic integration of catalase and Fe 3 O 4 magnetic nanoparticles with metal organic framework for colorimetric detection of phenol

Toxic phenol pollutants pose a great threat to the environment, it is urgent to develop an efficient and recyclable method to monitor phenol. Herein, we reported the synthesis of catalase-Fe3O4@ZIF-8 (CAT-Fe3O4@ZIF-8) through a novel amino-acid-boosted one-pot embedding strategy that synergically integrated catalase and magnetic Fe3O4 nanoparticles with ZIF-8. As expected, CAT-Fe3O4@ZIF-8 exhibited enhanced catalytic activity compared …

Synergetic integration of catalase and Fe 3 O 4 magnetic nanoparticles with metal organic framework for colorimetric detection of phenol Read More »

3D origami paper-based ratiometric fluorescent microfluidic device for visual point-of-care detection of alkaline phosphatase and butyrylcholinesterase

On-site multiplex enzyme detection is crucial for diagnosis, therapeutics and prognostic. To date, it is still a daunting challenge to develop portable, low-cost, and efficient multi-enzyme detection methods. Herein, a novel sample-in-result-out platform integrating ratiometric fluorescent assays with 3D origami microfluidic paper-based device (μPAD) was developed for simultaneous visual point-of-care testing (POCT) of alkaline phosphatase …

3D origami paper-based ratiometric fluorescent microfluidic device for visual point-of-care detection of alkaline phosphatase and butyrylcholinesterase Read More »

Binding of Cu+ and Cu2+ with peptides: Peptides = oxytocin, Arg8 -vasopressin, bradykinin, angiotensin-I, substance-P, somatostatin, and neurotensin.

The intrinsic binding ability of 7 natural peptides (oxytocin, arg8 -vasopressin, bradykinin, angiotensin-I, substance-P, somatostatin, and neurotensin) with copper in 2 different oxidation states (CuI/II ) derived from different Cu+/2+ precursor sources have been investigated for their charge-dependent binding characteristics. The peptide-CuI/II complexes, [M – (n-1)H + nCuI ] and [M – (2n-1)H + nCuII …

Binding of Cu+ and Cu2+ with peptides: Peptides = oxytocin, Arg8 -vasopressin, bradykinin, angiotensin-I, substance-P, somatostatin, and neurotensin. Read More »

National surveillance for non-polio enteroviruses in Canada: Why is it important?

National surveillance for non-polio enteroviruses in Canada: Why is it important?

A widespread outbreak of enterovirus D68 (EV-D68) was detected in affiliation with respiratory sickness in kids throughout Canada and the United States throughout the autumn of 2014. The majority of instances have been delicate, however some have been related to extra extreme sickness requiring hospitalization; among the instances additionally had neurological signs together with paralysis, …

National surveillance for non-polio enteroviruses in Canada: Why is it important? Read More »

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