Viral taxonomy derived from evolutionary genome relationships.

Ceruloplasmin Deficiency Impaired Brain Iron Metabolism and Behavior in Mice

Iron accumulation is an important cause of various brain diseases. As a ferroxidase, ceruloplasmin (Cp) plays a key role in iron homeostasis and its abnormal activity leads to iron accumulation. However, the detailed biological function of Cp in brain iron homeostasis needs to be investigated. In this study, Cp knockout mice were prepared and the …

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A simple colorimetric method for viable bacteria detection based on cell counting Kit-8

In this study, Cell Counting Kit-8 (CCK-8) was introduced to detect the concentration of live bacteria for the first time depending on the redox reaction between CCK-8 solution and dehydrogenase. CCK-8 solution can be reduced to form water soluble orange-yellow formazan by the dehydrogenase present in bacterial cells, and the concentration of live bacteria is …

A simple colorimetric method for viable bacteria detection based on cell counting Kit-8 Read More »

Efficient immobilization of catalase on mesoporous MIL-101 (Cr) and its catalytic activity assay

Enzyme immobilization using metal-organic frameworks (MOFs) as carriers has aroused significant interest owing to the unique pore structure and versatile surface functional groups of MOFs. Catalase (CAT) is an important industrial enzyme that is widely used in the catalytic decomposition of hydrogen peroxide in the fields of food and biological products. In this study, mesoporous …

Efficient immobilization of catalase on mesoporous MIL-101 (Cr) and its catalytic activity assay Read More »

Pandora box of BCA assay. Investigation of the accuracy and linearity of the microplate bicinchoninic protein assay: Analytical challenges and method modifications to minimize systematic errors

Bicinchoninic colorimetric assay is very widely used for total protein quantitative analysis. We report that bicinchoninic (BCA) total protein assay linearity range and the assay sensitivity are counterbalancing factors. BCA assay true linear range may be considerably narrower than the manufacturer’s advertised 20-2000 μg/ml and therefore the choice of the assay calibration range should not …

Pandora box of BCA assay. Investigation of the accuracy and linearity of the microplate bicinchoninic protein assay: Analytical challenges and method modifications to minimize systematic errors Read More »

Pandora box of BCA assay. Investigation of the accuracy and linearity of the microplate bicinchoninic protein assay: Analytical challenges and method modifications to minimize systematic errors

Bicinchoninic colorimetric assay is very widely used for total protein quantitative analysis. We report that bicinchoninic (BCA) total protein assay linearity range and the assay sensitivity are counterbalancing factors. BCA assay true linear range may be considerably narrower than the manufacturer’s advertised 20-2000 μg/ml and therefore the choice of the assay calibration range should not …

Pandora box of BCA assay. Investigation of the accuracy and linearity of the microplate bicinchoninic protein assay: Analytical challenges and method modifications to minimize systematic errors Read More »

Viral taxonomy derived from evolutionary genome relationships.

Viral taxonomy derived from evolutionary genome relationships.

We describe a brand new genome alignment-based mannequin for understanding the range of viruses based mostly on evolutionary genetic relationships. This strategy makes use of data principle and a bodily mannequin to find out the knowledge shared by the genes in two genomes. Pairwise comparisons of genes from the viruses are created from alignments utilizing NCBI BLAST, …

Viral taxonomy derived from evolutionary genome relationships. Read More »

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